The Boss Mode is a brand new mode that is unlocked by defeating the Subspace Emissary. It is essentially a duplicate of the All-Star mode. However, instead of fighting the other playable characters, you have to fight the various bosses that are throughout the game

Unlike previous modes, you do not get any continues and so if you die, you have to start over. As with All-Star Mode there are three Heart Containers each of which heal all of your damage. There are only 10 stages. The first nine are always randomised, however the final one is always Tabuu and ironically is the hardest

 Petey Pirhana
Game Series: Super Mario Bros.
First Appearance: Super Mario Sunshine (2002)

Details:
The first boss we'll cover is Petey Pirhana. The fight with Petey puts you on a small platform. Petey is holding two cages, each with their seperate health bars. Petey will attack you with these cages and occassionally jump up and slam down in order to cause damage. If you are able to damage Petey himself, the damage is spread across both cages. Break one cage and the boss fight is over

Petey Pirhana
 Ridley
Game Series: Metroid
First Appearance: Metroid (1987)

Details:
The next Boss is Ridley. The flying dragon from the Metroid series appears as a Boss on a long platform in a base. Ridley has a variety of flying attacks involving flying across the screen at a high speed or towards the screen from ther background. Both of which do massive damage. Ridley also hits you with his tail and is known to scrape along the stage, taking you with it and causing decent damage. However Ridley has a lot of time spent where he isn't doing anything. use this time to attack

Ridley
 Duon
Game Series: Smash Bros.
First Appearance: Super Smash Bros. Brawl (2008)

Details:
Duon is a rather formidable boss. It is a robot that runs off a wheel but has two distinct personalities. One of them is for physical combat, the other is more weapon based.. The Blue side is the more physical side and will attack you with a variety of attacks including a headbutt. The Pink side often fires misisles or bombs out. Duon will choose its attacks by whatever side is facing you so choose your tactics based upon your character's strength

Duon
 Rayquaza
Game Series: Pokémon
First Appearance: Pokémon Ruby/Sapphire (2002)

Details:
When you fight Rayquaza, you are in a Jungle and as such cannot be knocked down. However, Rayquaza has a variety of powerful attacks that can easily send you flying upwards so you have to be careful. It uses its tail to attack you when you're nearby, it sends out a variety of energy pulses at you. It also has many flight based attacks. It will rush you from the side causing damage or fly up and then down into the ground. After it has done so, it will come out at another point. Make sure you're out of the way yet close enough to attack within this short time. If Rayquaza starts spinning, he will send a Thunder attack down on you. However, like Ridley, he has a lot of time where he's just floating there, open for attack

Rayquaza
 Master Hand
Game Series: Smash Bros.
First Appearance: Super Smash Bros. (1999)

Details:
Master Hand, the classic boss of the Super Smash Bros. series appears in Boss Mode as well as in Classic Mode. Fighting upon the Final Destination map, Master Hand takes up position on the right. Like previous games, he has a variety of attacks. He can shoot bullets at you, which can be reflected with the right characters. He can grab you and squeeze you to cause damage. He slams down on the stage either with the palm, fist or rotating fingers to cause decent damage if you are caught within.

Master Hand
 Galleom
Game Series: Smash Bros.
First Appearance: Super Smash Bros. Brawl (2008)

Details:
Galleom is another boss unique to Smash Bros. He is a very large robot and as such, when he attacks, he will cause significant damage to any character he hits. He has rocket launchers for distanced attacks and he also jumps up and slams down repeatedly to catch you underneath. However these can be easily dodged. He also sometimes converts into a vehicle and quickly moves around the stage. If you are hit by him, you will receive damage

Galleom
 Meta-Ridley
Game Series: Metroid
First Appearance: Metroid Prime (2003)

Details:
Meta-Ridley is essentially another fight against Ridley. However, unlike the past one, Ridley is constantly in flight and stays towards the left of the stage while attacking. This time, Ridley uses some projectile flames against you and is even known to Slam the stage down off the screen causing you to have to jump in order to survive. When Meta-Ridley starts getting close to dying, he will stay further off the stage so you have to jump into him to attack as opposed to attacking beneath him

Meta-Ridley
 Crazy Hand
Game Series: Smash Bros.
First Appearance: Super Smash Bros. Melee (2001)

Details:
Crazy Hand is like you expect, a crazier version of Master Hand. Taking up the opposite side of the stage, Crazy Hand's attacks are similar, just quicker and more in quantity. He also has his own unique attacks of walking along the stage to flick you, dropping bombs on the stage and playing dead before attacking you quickly. It's best to take Crazy Hand out as quickly as possible as you do not know whats coming next. Like the other bosses though, there are times of no activity leaving it open for attack

Crazy Hand
 Porky
Game Series: Earthbound
First Appearance: Mother 3 (2006)

Details:
Porky appears in Brawl inside a massive Spider-like mecha. When to the side of it, Porky will thrash at you with his legs. He also charges up laser shots from the top of the Mecha that cause decent damage. Sometimes he sends out some robots that while they wont damage you as they walk past, they will explode. Porky also jumps up and slams down to cause decent damge. You can however walk on and to the sides of the middle of the mecha to get the most damage in as possible

Porky
 Tabuu
Game Series: Smash Bros.
First Appearance: Super Smash Bros. Brawl (2008)

Details:
Tabuu is the final boss in the Subpsace Emissary and the final boss in Boss Mode. Like the other bosses, Tabuu has a plethora of attacks. However, his position on the stage is not constant and he'll be continually teleporting to a new area. Among his attacks are him getting a sharp sword style ribbon in front of him and charging along the stage. He can jump up and shoot lasers, grow really big to blow you away with two continual lasers and his teleporting also damages. The most difficult of his attacks to evade however is when he gets his wings, he will send out three massive pulses that encompass the entire stage. The only way to dodge them is by using your sheild just as its about to hit. This requires decent timing, but if you're off by a split second, chances are you wont have another chance to evade

Tabuu
 
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